Gene description for FLOT2
Gene name	flotillin 2
Gene symbol	FLOT2
Other names/aliases	ECS-1 ECS1 ESA ESA1 M17S1
Species	Homo sapiens
Experiment description of studies that identified FLOT2 in Embryonic kidney cells
1 Experiment ID 925 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Mass spectrometry PubMed ID 25483805    Organism Homo sapiens Experiment description Vertebrate Hedgehog is secreted on two types of extracellular vesicles with different signaling properties Authors Vyas N, Walvekar A, Tate D, Lakshmanan V, Bansal D, Lo Cicero A, Raposo G, Palakodeti D, Dhawan J. Journal name Sci Rep Publication year 2014 Sample Embryonic kidney cells Sample name HEK293T - P150 - Fraction 1.12 g/mL Isolation/purification methods Differential centrifugation Ultracentrifugation Density gradient centrifugation Flotation density 1.09-1.12 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting EV-TRACK EV140018: EV-METRIC:56%, 56%
2 Experiment ID 876 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Western blotting PubMed ID 29955024    Organism Homo sapiens Experiment description Essential Role of Sphingosine Kinase 2 in the Regulation of Cargo Contents in the Exosomes from K562 Cells Authors Mohamed NNI, Okada T, Kajimoto T, Nakamura SI. Journal name Kobe J Med Sci Publication year 2018 Sample Embryonic kidney cells Sample name HEK293T - control siRNA transfected Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
3 Experiment ID 877 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Western blotting PubMed ID 29955024    Organism Homo sapiens Experiment description Essential Role of Sphingosine Kinase 2 in the Regulation of Cargo Contents in the Exosomes from K562 Cells Authors Mohamed NNI, Okada T, Kajimoto T, Nakamura SI. Journal name Kobe J Med Sci Publication year 2018 Sample Embryonic kidney cells Sample name HEK293T - SphK1 siRNA transfected Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
4 Experiment ID 878 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Western blotting PubMed ID 29955024    Organism Homo sapiens Experiment description Essential Role of Sphingosine Kinase 2 in the Regulation of Cargo Contents in the Exosomes from K562 Cells Authors Mohamed NNI, Okada T, Kajimoto T, Nakamura SI. Journal name Kobe J Med Sci Publication year 2018 Sample Embryonic kidney cells Sample name HEK293T - SphK2 siRNA transfected Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
5 Experiment ID 922 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Western blotting PubMed ID 25483805    Organism Homo sapiens Experiment description Vertebrate Hedgehog is secreted on two types of extracellular vesicles with different signaling properties Authors Vyas N, Walvekar A, Tate D, Lakshmanan V, Bansal D, Lo Cicero A, Raposo G, Palakodeti D, Dhawan J. Journal name Sci Rep Publication year 2014 Sample Embryonic kidney cells Sample name HEK293T - P150 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Immunofluorescence EV-TRACK EV140018: EV-METRIC:56%, 56%
6 Experiment ID 1925 Identified molecule protein Extracellular vesicle type Small extracellular vesicles MISEV Small EVs Identification method Western blotting PubMed ID 36214496    Organism Homo sapiens Experiment description Contaminating transfection complexes can masquerade as small extracellular vesicles and impair their delivery of RNA Authors McCann J, Sosa-Miranda CD, Guo H, Reshke R, Savard A, Zardini Buzatto A, Taylor JA, Li L, Gibbings DJ. Journal name J Extracell Vesicles Publication year 2022 Sample Embryonic kidney cells Sample name HEK293 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
7 Experiment ID 1926 Identified molecule protein Extracellular vesicle type Small extracellular vesicles MISEV Small EVs Identification method Western blotting PubMed ID 36214496    Organism Homo sapiens Experiment description Contaminating transfection complexes can masquerade as small extracellular vesicles and impair their delivery of RNA Authors McCann J, Sosa-Miranda CD, Guo H, Reshke R, Savard A, Zardini Buzatto A, Taylor JA, Li L, Gibbings DJ. Journal name J Extracell Vesicles Publication year 2022 Sample Embryonic kidney cells Sample name HEK293 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
8 Experiment ID 1927 Identified molecule protein Extracellular vesicle type Small extracellular vesicles MISEV Small EVs Identification method Western blotting PubMed ID 36214496    Organism Homo sapiens Experiment description Contaminating transfection complexes can masquerade as small extracellular vesicles and impair their delivery of RNA Authors McCann J, Sosa-Miranda CD, Guo H, Reshke R, Savard A, Zardini Buzatto A, Taylor JA, Li L, Gibbings DJ. Journal name J Extracell Vesicles Publication year 2022 Sample Embryonic kidney cells Sample name HEK293 - fraction 2 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density 1.05 g/mL Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
9 Experiment ID 1928 Identified molecule protein Extracellular vesicle type Small extracellular vesicles MISEV Small EVs Identification method Western blotting PubMed ID 36214496    Organism Homo sapiens Experiment description Contaminating transfection complexes can masquerade as small extracellular vesicles and impair their delivery of RNA Authors McCann J, Sosa-Miranda CD, Guo H, Reshke R, Savard A, Zardini Buzatto A, Taylor JA, Li L, Gibbings DJ. Journal name J Extracell Vesicles Publication year 2022 Sample Embryonic kidney cells Sample name HEK293 - fraction 3 Isolation/purification methods Differential centrifugation Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
10 Experiment ID 1929 Identified molecule protein Extracellular vesicle type Small extracellular vesicles MISEV Small EVs Identification method Western blotting PubMed ID 36214496    Organism Homo sapiens Experiment description Contaminating transfection complexes can masquerade as small extracellular vesicles and impair their delivery of RNA Authors McCann J, Sosa-Miranda CD, Guo H, Reshke R, Savard A, Zardini Buzatto A, Taylor JA, Li L, Gibbings DJ. Journal name J Extracell Vesicles Publication year 2022 Sample Embryonic kidney cells Sample name HEK293 - fraction 4 Isolation/purification methods Differential centrifugation Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
11 Experiment ID 1930 Identified molecule protein Extracellular vesicle type Small extracellular vesicles MISEV Small EVs Identification method Western blotting PubMed ID 36214496    Organism Homo sapiens Experiment description Contaminating transfection complexes can masquerade as small extracellular vesicles and impair their delivery of RNA Authors McCann J, Sosa-Miranda CD, Guo H, Reshke R, Savard A, Zardini Buzatto A, Taylor JA, Li L, Gibbings DJ. Journal name J Extracell Vesicles Publication year 2022 Sample Embryonic kidney cells Sample name HEK293 - fraction 5 Isolation/purification methods Differential centrifugation Ultracentrifugation OptiPrep density gradient centrifugation Flotation density 1.2 g/mL Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
12 Experiment ID 1932 Identified molecule protein Extracellular vesicle type Small extracellular vesicles MISEV Small EVs Identification method Western blotting PubMed ID 36214496    Organism Homo sapiens Experiment description Contaminating transfection complexes can masquerade as small extracellular vesicles and impair their delivery of RNA Authors McCann J, Sosa-Miranda CD, Guo H, Reshke R, Savard A, Zardini Buzatto A, Taylor JA, Li L, Gibbings DJ. Journal name J Extracell Vesicles Publication year 2022 Sample Embryonic kidney cells Sample name HEK293 - fraction 3-4 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
13 Experiment ID 1408 Identified molecule Protein Extracellular vesicle type Extracellular vesicles MISEV Small EVs Identification method Mass spectrometry PubMed ID 36706192    Organism Homo sapiens Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles Authors Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q. Journal name Sci Adv Publication year 2023 Sample Embryonic kidney cells Sample name HEK293T Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry EV-TRACK -
14 Experiment ID 1413 Identified molecule Protein Extracellular vesicle type Extracellular vesicles MISEV Small EVs Identification method Mass spectrometry PubMed ID 36706192    Organism Homo sapiens Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles Authors Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q. Journal name Sci Adv Publication year 2023 Sample Embryonic kidney cells Sample name HEK293T - Fraction 5 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation Flotation density 1.151 g/mL Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry EV-TRACK -
15 Experiment ID 1659 Identified molecule Protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Mass spectrometry PubMed ID 34108659    Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. Journal name Nat Cell Biol Publication year 2021 Sample Embryonic kidney cells Sample name HEK293T Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein mRNA Methods used in the study RT-qPCR Western blotting Mass spectrometry Flow cytometry EV-TRACK -
16 Experiment ID 1671 Identified molecule Protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Mass spectrometry PubMed ID 34108659    Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. Journal name Nat Cell Biol Publication year 2021 Sample Embryonic kidney cells Sample name HEK293T - Exo-rich fractions 7-10 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry EV-TRACK -
17 Experiment ID 1671 Identified molecule Protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Mass spectrometry PubMed ID 34108659    Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. Journal name Nat Cell Biol Publication year 2021 Sample Embryonic kidney cells Sample name HEK293T - Exo-rich fractions 7-10 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry EV-TRACK -
18 Experiment ID 1672 Identified molecule Protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Mass spectrometry PubMed ID 34108659    Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. Journal name Nat Cell Biol Publication year 2021 Sample Embryonic kidney cells Sample name HEK293T - Exo-rich fractions 1-6 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK -
19 Experiment ID 1672 Identified molecule Protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Mass spectrometry PubMed ID 34108659    Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. Journal name Nat Cell Biol Publication year 2021 Sample Embryonic kidney cells Sample name HEK293T - Exo-rich fractions 1-6 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK -